Part:BBa_K4833022

The asdA gene encodes an enzyme essential for the synthesis of diaminopimelic acid (DAP).

The asdA gene encodes an enzyme essential for the synthesis of diaminopimelic acid (DAP). To compensate for the lack of the asdA mutation in the Salmonella genome, the asd sequence is inserted into a recombinant plasmid and placed under the control of the Arabidose operon. This allows bacteria to synthesize DAP for growth when Arabidose is present.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 439
  

Effect of asdA gene in plasmid on bacterial growth

Fig.1 Bacteria do not grow on LB agar plate in the absence of plasmid.

Fig.2 Bacteria grow normally on LB agar plate in the presence of plasmid.